This Technical Specification specifies a method to evaluate the effects of pollutants on spore germination of a mycorrhizal fungus, Glomus mosseae. This direct acute toxicity bioassay allows the evaluation of potential effects of pollutants and contaminated soils on beneficial soil microorganisms important for plant growth within the concept of sustainable agriculture. This Technical Specification is applicable to ⎯ chemical substances, and ⎯ contaminated soils, waste and soil-waste mixture and sludge.