This Gulf Standard specifies a method for the gas-chromatographic determination of nicotine in cigarette smoke condensates. The smoking of cigarettes and the collection of mainstream smoke are normally carried out in accordance with GSO 644. However, the method specified in this International Standard is also applicable to the determination of nicotine in cigarette smoke condensates obtained by non standard smoking. NOTE In countries not in a position to use the gas-chromatographic method, reference should be made to GSO 101 for the determination of total nicotine alkaloids. In such cases, values obtained using the method described in GSO 101 may be used with the addition of a note in the expression of results.